2003 issue 3

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Volume 12, issue 3

Review article

The application of c-Fos-immunoreactivity in neuropsychopharmacology

ALEKSANDRA WISŁOWSKA2, MAŁGORZATA LEHNER1, EWA TARACHA1, MAŁGORZATA ZIENOWICZ2, Adam Płaźnik1,2
1. Zakład Neurochemii Instytutu Psychiatrii i Neurologii w Warszawie
2. Katedra i Zakład Farmakologii Doświadczalnej i Klinicznej w Warszawie
Postępy Psychiatrii i Neurologii, 2003, 12 (3), 335-345
Keywords: c-Fos protein, antidepressants, drugs of abuse, antipsychotics, anxiety, learning and memory

Abstract

Aims – The article presents new data concerning the c-Fos-immunoreactivity technique application to localization of the CNS structures activated by such substances as antidepressants, narcoleptics, or habit-forming substances, as well as to mapping of pathways implicated in pain transmission, anxiety and learning.

Review – C-Fos protein is a product of c-fos gene – a member or the immediate early gene family (IEG). The basal level of c-fos mRNA is hardly detectable in non-stimulated cells, but its expression increases rapidly on administration or various stimuli. Fos immunochemistry offers a mapping tool enabling the investigation or functional neural activity (the so-called a transcriptional activity) in the brain.

Conclusions – Despite certain limitations, the technique of c-Fos protein mapping proved to be helpful in both in neurobiological and neuropharmacological studies investigating the central nervous system functioning in physiological and pathological states.

Address for correspondence:
Dr Ewa Taracha, Zakład Neurochemii Instytutu Psychiatrii i Neurologii,
ul. Sobieskiego 9, 02-957 Warszawa, tel. (22) 3213319,.fax: 3213471, e-mail: taracha@ipin.edu.pl